FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7178658
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1025681.4287907294093132Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG572470.7974610296242No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC87150.12140152100852275Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21650.043.16269318
CGCCGTA233350.041.82961347
TCGCCGT235100.041.51824646
CCGTATC213650.041.41201449
GGTCGCC258700.041.2075344
GTCGCCG251450.040.47490745
GTGGTCG261900.040.30370742
CTCGGTG213350.040.1423338
CGTATCA222650.040.11530750
GCCGTAT252900.039.7313748
TGGTCGC265650.039.6030243
TCTCGGT214400.039.50552737
ATCTCGG216500.039.4133336
GGTGGTC268850.039.0925941
AGAGCAC43550.038.81684515
GATCTCG212800.038.81572335
AGCACAC36350.038.60958517
GAGCACA41800.038.34815216
TCGGTGG254750.038.30282239
AGATCTC224800.037.1804334