Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 7178658 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 102568 | 1.4287907294093132 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 57247 | 0.7974610296242 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 8715 | 0.12140152100852275 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 2165 | 0.0 | 43.162693 | 18 |
CGCCGTA | 23335 | 0.0 | 41.829613 | 47 |
TCGCCGT | 23510 | 0.0 | 41.518246 | 46 |
CCGTATC | 21365 | 0.0 | 41.412014 | 49 |
GGTCGCC | 25870 | 0.0 | 41.20753 | 44 |
GTCGCCG | 25145 | 0.0 | 40.474907 | 45 |
GTGGTCG | 26190 | 0.0 | 40.303707 | 42 |
CTCGGTG | 21335 | 0.0 | 40.14233 | 38 |
CGTATCA | 22265 | 0.0 | 40.115307 | 50 |
GCCGTAT | 25290 | 0.0 | 39.73137 | 48 |
TGGTCGC | 26565 | 0.0 | 39.60302 | 43 |
TCTCGGT | 21440 | 0.0 | 39.505527 | 37 |
ATCTCGG | 21650 | 0.0 | 39.41333 | 36 |
GGTGGTC | 26885 | 0.0 | 39.09259 | 41 |
AGAGCAC | 4355 | 0.0 | 38.816845 | 15 |
GATCTCG | 21280 | 0.0 | 38.815723 | 35 |
AGCACAC | 3635 | 0.0 | 38.609585 | 17 |
GAGCACA | 4180 | 0.0 | 38.348152 | 16 |
TCGGTGG | 25475 | 0.0 | 38.302822 | 39 |
AGATCTC | 22480 | 0.0 | 37.18043 | 34 |