FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9175574
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4861395.298186249710373Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1946442.1213277774229713No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC358500.39071125141598767Illumina Single End PCR Primer 1 (98% over 50bp)
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA159910.17427792528293054No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC119670.13042235831785565Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC113900.12413392339269456Illumina Single End PCR Primer 1 (100% over 49bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC96910.10561737064079042Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC917850.046.3660249
GTGACTG56850.046.3526731
CGTATCA931250.045.99574350
CGCCGTA1000250.045.95807647
TCGCCGT1000400.045.6853146
GTATCAT880900.045.65323651
GTCGCCG1061750.044.729945
GCCGTAT1046250.044.68006548
CTCGGTG912500.044.64926538
GGTCGCC1095300.044.57398644
TCTCGGT908350.044.4766337
GTGGTCG1090700.044.32582542
GGTGGTC1090200.044.2273841
GATCTCG903100.044.18486435
ATCTCGG924000.044.1778636
TGACTGG59850.044.1176832
TGGTCGC1092600.043.96686643
CATTAAA977950.043.95009655
TCGGTGG1047950.043.8027339
AGATCTC921000.043.5695634