Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-19.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9175574 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 486139 | 5.298186249710373 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 194644 | 2.1213277774229713 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 35850 | 0.39071125141598767 | Illumina Single End PCR Primer 1 (98% over 50bp) |
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA | 15991 | 0.17427792528293054 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC | 11967 | 0.13042235831785565 | Illumina Single End PCR Primer 1 (98% over 50bp) |
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC | 11390 | 0.12413392339269456 | Illumina Single End PCR Primer 1 (100% over 49bp) |
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC | 9691 | 0.10561737064079042 | Illumina Single End PCR Primer 1 (100% over 49bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTATC | 91785 | 0.0 | 46.36602 | 49 |
GTGACTG | 5685 | 0.0 | 46.352673 | 1 |
CGTATCA | 93125 | 0.0 | 45.995743 | 50 |
CGCCGTA | 100025 | 0.0 | 45.958076 | 47 |
TCGCCGT | 100040 | 0.0 | 45.68531 | 46 |
GTATCAT | 88090 | 0.0 | 45.653236 | 51 |
GTCGCCG | 106175 | 0.0 | 44.7299 | 45 |
GCCGTAT | 104625 | 0.0 | 44.680065 | 48 |
CTCGGTG | 91250 | 0.0 | 44.649265 | 38 |
GGTCGCC | 109530 | 0.0 | 44.573986 | 44 |
TCTCGGT | 90835 | 0.0 | 44.47663 | 37 |
GTGGTCG | 109070 | 0.0 | 44.325825 | 42 |
GGTGGTC | 109020 | 0.0 | 44.22738 | 41 |
GATCTCG | 90310 | 0.0 | 44.184864 | 35 |
ATCTCGG | 92400 | 0.0 | 44.17786 | 36 |
TGACTGG | 5985 | 0.0 | 44.117683 | 2 |
TGGTCGC | 109260 | 0.0 | 43.966866 | 43 |
CATTAAA | 97795 | 0.0 | 43.950096 | 55 |
TCGGTGG | 104795 | 0.0 | 43.80273 | 39 |
AGATCTC | 92100 | 0.0 | 43.56956 | 34 |