FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7104782
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC491960.6924350388231476Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316410.44534793608023443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA105400.044.76109747
GGTCGCC116850.044.0590844
TCGCCGT105700.043.77316346
CCGTATC105350.043.42057449
GTCGCCG117150.043.28930345
CTCGGTG109950.042.337338
GTGGTCG122250.041.8555542
GCCGTAT118450.041.4845748
TCTCGGT112450.041.30325737
ATCTCGG115250.041.1501336
CGTATCA114000.040.52476550
GATCTCG115950.040.23761735
TGGTCGC128200.039.28504643
TCGGTGG133200.039.07160639
GGTGGTC133200.038.44099841
AGATCTC122800.037.7661434
GCACACG16050.037.28803318
TGTAGGG149550.036.9993318
GTAGGGA148500.036.88359519
CGTGTAG150750.036.70480316
Produced by FastQC (version 0.11.5)