FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8964776
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1817372.0272341439429162Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG764710.853016293993291No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC153010.17067911122374949Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA370550.046.51651847
TCGCCGT372600.046.1290946
CCGTATC346850.046.06243549
GGTCGCC410350.045.16055744
GTCGCCG397350.045.11429245
CGTATCA357550.044.98741550
CTCGGTG340800.044.59030538
GTGGTCG412600.044.54131342
GCCGTAT394550.044.3613848
ATCTCGG344800.044.1138636
TCTCGGT342200.043.9685937
GATCTCG339300.043.71492435
GGTGGTC420950.043.51620541
TGGTCGC422150.043.26009843
TCGGTGG401150.043.23866739
CGGTGGT423600.042.14510740
AGATCTC356250.042.03780734
GTATCAT356150.042.00983451
TAGATCT382000.040.77080533
GTAGGGA481850.040.4356519