Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-17.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8964776 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 181737 | 2.0272341439429162 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 76471 | 0.853016293993291 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 15301 | 0.17067911122374949 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCCGTA | 37055 | 0.0 | 46.516518 | 47 |
TCGCCGT | 37260 | 0.0 | 46.12909 | 46 |
CCGTATC | 34685 | 0.0 | 46.062435 | 49 |
GGTCGCC | 41035 | 0.0 | 45.160557 | 44 |
GTCGCCG | 39735 | 0.0 | 45.114292 | 45 |
CGTATCA | 35755 | 0.0 | 44.987415 | 50 |
CTCGGTG | 34080 | 0.0 | 44.590305 | 38 |
GTGGTCG | 41260 | 0.0 | 44.541313 | 42 |
GCCGTAT | 39455 | 0.0 | 44.36138 | 48 |
ATCTCGG | 34480 | 0.0 | 44.11386 | 36 |
TCTCGGT | 34220 | 0.0 | 43.96859 | 37 |
GATCTCG | 33930 | 0.0 | 43.714924 | 35 |
GGTGGTC | 42095 | 0.0 | 43.516205 | 41 |
TGGTCGC | 42215 | 0.0 | 43.260098 | 43 |
TCGGTGG | 40115 | 0.0 | 43.238667 | 39 |
CGGTGGT | 42360 | 0.0 | 42.145107 | 40 |
AGATCTC | 35625 | 0.0 | 42.037807 | 34 |
GTATCAT | 35615 | 0.0 | 42.009834 | 51 |
TAGATCT | 38200 | 0.0 | 40.770805 | 33 |
GTAGGGA | 48185 | 0.0 | 40.43565 | 19 |