FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8275585
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC453930.5485171138958757Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG321830.3888909364111419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC107800.043.14727444
CGCCGTA99700.043.03678547
GCACACG21950.042.5727618
TCGCCGT101250.042.20537646
GTCGCCG108050.041.7518145
CTCGGTG97750.040.60285638
TCTCGGT98800.039.9951937
GTGGTCG116650.039.84449842
ATCTCGG100500.039.59702736
CCGTATC100550.039.50580649
GCCGTAT113150.038.9115548
TGGTCGC118300.038.63789443
GATCTCG102250.037.9608935
TCGGTGG119450.037.15228339
CGTATCA109300.036.7914650
GGTGGTC127150.036.08641
CGGTGGT131350.034.63902740
AGATCTC113150.034.42817734
CGTGTAG143600.034.4142216
GTAGGGA144600.033.74034519
Produced by FastQC (version 0.11.5)