Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-15.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8204561 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 139198 | 1.696592907286569 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 58281 | 0.7103487925801271 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 11220 | 0.1367532034925452 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCCGTA | 32525 | 0.0 | 43.06329 | 47 |
CCGTATC | 29910 | 0.0 | 42.89666 | 49 |
TCGCCGT | 32795 | 0.0 | 42.4633 | 46 |
GGTCGCC | 35645 | 0.0 | 42.121468 | 44 |
GCACACG | 2020 | 0.0 | 42.102474 | 18 |
CGTATCA | 30825 | 0.0 | 41.963947 | 50 |
GTCGCCG | 34620 | 0.0 | 41.882763 | 45 |
GCCGTAT | 35070 | 0.0 | 41.035976 | 48 |
CTCGGTG | 29460 | 0.0 | 40.822006 | 38 |
GTGGTCG | 36525 | 0.0 | 40.570778 | 42 |
TGGTCGC | 36430 | 0.0 | 40.513256 | 43 |
ATCTCGG | 29890 | 0.0 | 40.21107 | 36 |
TCTCGGT | 29620 | 0.0 | 40.164284 | 37 |
GATCTCG | 29390 | 0.0 | 40.12109 | 35 |
GGTGGTC | 37110 | 0.0 | 39.506824 | 41 |
TCGGTGG | 34720 | 0.0 | 39.393772 | 39 |
GTATCAT | 31430 | 0.0 | 38.55051 | 51 |
AGATCTC | 30920 | 0.0 | 38.464058 | 34 |
CGGTGGT | 36790 | 0.0 | 38.280792 | 40 |
TAGATCT | 33150 | 0.0 | 37.396957 | 33 |