FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8204561
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1391981.696592907286569Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG582810.7103487925801271No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC112200.1367532034925452Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA325250.043.0632947
CCGTATC299100.042.8966649
TCGCCGT327950.042.463346
GGTCGCC356450.042.12146844
GCACACG20200.042.10247418
CGTATCA308250.041.96394750
GTCGCCG346200.041.88276345
GCCGTAT350700.041.03597648
CTCGGTG294600.040.82200638
GTGGTCG365250.040.57077842
TGGTCGC364300.040.51325643
ATCTCGG298900.040.2110736
TCTCGGT296200.040.16428437
GATCTCG293900.040.1210935
GGTGGTC371100.039.50682441
TCGGTGG347200.039.39377239
GTATCAT314300.038.5505151
AGATCTC309200.038.46405834
CGGTGGT367900.038.28079240
TAGATCT331500.037.39695733