FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8640960
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC839620.9716744435803429Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG480090.5555979891123208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA210550.039.92637347
TCGCCGT211700.039.21375346
CCGTATC199900.038.8502849
GCACACG24350.038.80806418
GGTCGCC231250.038.5317544
GTCGCCG226600.038.2569845
CGTATCA204750.038.08385550
GCCGTAT233950.037.60900548
CTCGGTG195350.037.58865438
ATCTCGG198100.037.1726436
GTGGTCG238500.036.98020642
TCTCGGT196550.036.95002737
TGGTCGC236500.036.8637743
GATCTCG198300.036.5527135
TCGGTGG232050.035.59488739
GGTGGTC248400.035.49226841
AGATCTC208900.034.8824534
GTAGGGA284950.034.51376319
GAGCACA42800.034.34493616
CGGTGGT252400.033.95913340
Produced by FastQC (version 0.11.5)