FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8313002
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1214031.460399023120649Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG662240.7966315898877444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA256150.044.487347
TCGCCGT258750.043.9323446
CCGTATC244300.043.76595349
GGTCGCC280900.043.5328644
GTCGCCG278250.043.0043745
CGTATCA252900.042.7481950
CTCGGTG246750.042.5381338
GTGGTCG285550.042.3342142
ATCTCGG249900.042.1847736
TGGTCGC283700.042.14148343
TCTCGGT247750.041.9436437
GCCGTAT275700.041.8790548
GATCTCG248400.041.6504635
GGTGGTC294350.040.91399841
TCGGTGG285150.040.8591339
AGATCTC259950.039.97489534
CGGTGGT304300.039.25415440
TAGATCT279150.038.62967333
CGTGTAG333250.038.5541116
TGTAGGG330850.038.53735718