FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8942471
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2997303.3517581438061135Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1315851.4714612996788023No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC254280.2843509361115065Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC572900.045.646749
CGCCGTA634200.045.30128547
CGTATCA582850.045.19771250
TCGCCGT635400.044.94032746
GTATCAT555500.044.07751551
GTCGCCG676950.043.99663545
GGTCGCC698650.043.8977344
CTCGGTG569700.043.71679738
GCCGTAT663650.043.71338348
TCTCGGT569000.043.482737
GTGGTCG702850.043.3966442
TGGTCGC698950.043.28326443
ATCTCGG577350.043.24180636
GATCTCG563500.043.21766735
GGTGGTC704900.043.02714541
TCGGTGG672100.042.62743439
AGATCTC583150.042.12774334
CGGTGGT706200.041.87746440
CATTAAA633500.041.860555
TAGATCT618700.041.64749533