FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6916903
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG787771.1389056634161272No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC339860.49134706674359896Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA104000.051.0164247
ATCTCGG129500.049.86427736
CCGTATC107700.049.62193749
GATCTCG129150.049.32191535
TCGCCGT103350.048.9668246
CTCGGTG131750.048.82604238
GCCGTAT128350.048.72770348
GGTCGCC134100.048.4648944
GTCGCCG136300.047.708345
TCTCGGT130050.047.63496437
CGTATCA121550.047.45145450
AGATCTC126000.046.49911534
GTGGTCG143500.046.19257742
GTAGGGA179700.046.0035819
TCGGTGG178050.045.30780839
TGGTCGC126800.044.9344343
TAGATCT139700.043.7679633
GGTGGTC152750.043.69317241
CGTGTAG213300.043.67943216
GAGTGTA203750.043.45898428
Produced by FastQC (version 0.11.5)