Basic Statistics
Measure | Value |
---|---|
Filename | HHGW2AFX2_n02_ncm11-10.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8105152 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 131803 | 1.6261632107577992 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 81338 | 1.0035345419802122 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC | 9477 | 0.1169256295255166 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCCGTA | 26785 | 0.0 | 46.294056 | 47 |
TCGCCGT | 26980 | 0.0 | 45.622192 | 46 |
CCGTATC | 25125 | 0.0 | 45.60562 | 49 |
GGTCGCC | 29955 | 0.0 | 44.73675 | 44 |
CGTATCA | 25770 | 0.0 | 44.6407 | 50 |
GTCGCCG | 29135 | 0.0 | 44.614426 | 45 |
GCCGTAT | 28385 | 0.0 | 44.436684 | 48 |
TGGTCGC | 29875 | 0.0 | 43.907635 | 43 |
CTCGGTG | 25385 | 0.0 | 43.74657 | 38 |
GTGGTCG | 30430 | 0.0 | 43.4631 | 42 |
ATCTCGG | 25725 | 0.0 | 42.978176 | 36 |
TCTCGGT | 25685 | 0.0 | 42.581818 | 37 |
GATCTCG | 25425 | 0.0 | 42.466385 | 35 |
TCGGTGG | 30450 | 0.0 | 41.813942 | 39 |
GGTGGTC | 31535 | 0.0 | 41.76256 | 41 |
GTATCAT | 25880 | 0.0 | 41.462578 | 51 |
CGGTGGT | 31965 | 0.0 | 40.71901 | 40 |
AGATCTC | 26750 | 0.0 | 40.415993 | 34 |
GAGTGTA | 33815 | 0.0 | 39.404274 | 28 |
GTAGGGA | 35480 | 0.0 | 39.299965 | 19 |