FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n02_ncm11-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n02_ncm11-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8105152
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1318031.6261632107577992Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG813381.0035345419802122No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC94770.1169256295255166Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA267850.046.29405647
TCGCCGT269800.045.62219246
CCGTATC251250.045.6056249
GGTCGCC299550.044.7367544
CGTATCA257700.044.640750
GTCGCCG291350.044.61442645
GCCGTAT283850.044.43668448
TGGTCGC298750.043.90763543
CTCGGTG253850.043.7465738
GTGGTCG304300.043.463142
ATCTCGG257250.042.97817636
TCTCGGT256850.042.58181837
GATCTCG254250.042.46638535
TCGGTGG304500.041.81394239
GGTGGTC315350.041.7625641
GTATCAT258800.041.46257851
CGGTGGT319650.040.7190140
AGATCTC267500.040.41599334
GAGTGTA338150.039.40427428
GTAGGGA354800.039.29996519