FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9567476
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA9136859.549906370290346TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344110.3596664365816021No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC300430.3140117623498611TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC241320.25222953263744796TruSeq Adapter, Index 14 (97% over 38bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT239790.25063036479004497TruSeq Adapter, Index 14 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC217630.22746856119628625TruSeq Adapter, Index 14 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG205790.21509330151442238TruSeq Adapter, Index 14 (97% over 37bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC176260.18422831685180083TruSeq Adapter, Index 14 (97% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGT146750.15338423634404727TruSeq Adapter, Index 14 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGATCGGAAGAGCACA132950.1389603694851181TruSeq Adapter, Index 13 (97% over 35bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAT131820.13777928473507536TruSeq Adapter, Index 2 (97% over 37bp)
GTGACTGGAGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125960.1316543673587475No Hit
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCG115230.12043928827205838TruSeq Adapter, Index 14 (97% over 38bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC112800.11789943345559477TruSeq Adapter, Index 14 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGT696800.064.657862
TCTCGGG701550.064.44485561
CGGGTGC706600.064.2005264
TTCTCGG706300.064.00741660
ATATGGG733000.063.69151733
TCGGGTG715550.063.59374263
GGGTGCC714650.063.58792565
ATTCTCG712350.063.45629559
TATATGG744800.062.982832
GTGCCAA726300.062.8367167
GGTGCCA724850.062.81101266
GCCAAGG725700.062.77570369
CCAAGGA707500.062.62812870
TCTTATA750800.062.61247629
CTTATAT751850.062.36334230
GGAATTC726800.062.28846756
GGCAGAC197550.061.921341
TTATATG759900.061.8631531
TGCCAAG735200.061.82299868
TATGGGT522850.061.71167434
Produced by FastQC (version 0.11.5)