FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8822141
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2702773.0636214043733827TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC230780.2615918290129346TruSeq Adapter, Index 9 (100% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC152780.17317791678913316TruSeq Adapter, Index 9 (98% over 50bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC143070.16217151822896506TruSeq Adapter, Index 9 (100% over 48bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC124390.14099751976305977TruSeq Adapter, Index 9 (100% over 50bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG95410.10814835083683201TruSeq Adapter, Index 9 (100% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT92860.1052578960141308TruSeq Adapter, Index 9 (100% over 48bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA34550.046.7253231
GTCGGAA41700.038.8736842
GCGTGAA137600.036.90644560
GCCGTCG127900.036.3943850
CGGCGTG62000.035.789158
AAAAGGG204400.035.5467370
GGCGTGA61850.035.3666259
GCGTGGA38450.034.86226760
CTGCGTG79800.034.82337658
TGCGTGA80800.034.60896759
CCGTCGT134550.034.43974751
TCGGCGT64300.034.34583357
CGTGAAA150650.034.2205461
GATCTCG616450.033.07697740
TATGCCG656350.032.9005347
ATGCCGT640200.032.88313348
CTCGTAT627500.032.81242443
TGCCGTC595300.032.79998849
GTATGCC660450.032.7545846
TCTCGTA623050.032.7490642
Produced by FastQC (version 0.11.5)