FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10254110
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTG1172281.1432293977731856TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTG728830.7107686576406923TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGG386430.37685376887901534TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATG179450.17500299879755532TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTT177080.17269173043784394TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTT172710.168430024643777TruSeq Adapter, Index 8 (97% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG524100.047.6403470
AGTCGGA39250.046.837961
GGGTGGA145750.045.07271660
TGGCGGG209150.042.9227549
TCGGGGA50850.042.8800342
CGGGGTT160350.042.5185143
CGGGGAT44700.042.515843
GTTGGCG94800.040.72163847
TCGGGGT183350.040.16269742
TTGGCGG104250.039.68253748
GGTGGAA167850.039.22161561
AAAAAAG705100.038.9451669
GGTTGGC107200.038.65582346
TGCCGGG48200.038.1215349
GTCGGAA48000.037.708262
GCGGGGT153300.037.4420651
GTGGAAA197300.037.00370862
TGGGGGT136700.035.97210357
CGGGGTG193250.034.97202752
GGGTGGC147750.034.37145646
Produced by FastQC (version 0.11.5)