FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7865481
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTG291070.37006001285871776TruSeq Adapter, Index 7 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTG259760.33025316569959295TruSeq Adapter, Index 7 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA72300.039.45207660
AAAAAGG264850.039.0752670
CGGGGTT64850.037.4004343
TGGCGGG104350.036.826549
TCGGGGT68700.036.67997742
AGTCGGA20400.035.1936451
CGGGGTG102650.034.43602452
CATCGGG209050.034.18683640
ATCGGGG182450.034.0492741
TCGGGGA19850.033.85275742
GTTGGCG52550.033.4334847
GCGGGGT83900.033.3717251
TGCCGGG32700.032.85800649
GGTTGGC60750.032.6079546
TTGGCGG63250.032.0382448
GGTGGAA92900.031.7963561
AAAAAAG355050.031.35629569
ATCGCGG30750.031.18593641
TCATCGG233700.031.14979439
GATCATC318300.030.8203137
Produced by FastQC (version 0.11.5)