FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8705013
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2416852.776388731412578TruSeq Adapter, Index 6 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTG285880.328408469924169TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATG263190.30234302924073747TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC174730.2007234222395762TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGGATG103430.11881659453007135TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATT101310.11638121620266391TruSeq Adapter, Index 6 (97% over 48bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC92130.10583556853964492TruSeq Adapter, Index 6 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTGTG91610.10523821159141289TruSeq Adapter, Index 6 (97% over 49bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC90770.10427325036734579TruSeq Adapter, Index 6 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA36550.048.292941
GGGTGGA128550.042.60820460
GCGTGGA102100.041.7515460
GCCGTCG148100.039.96129250
GTGGAAA251750.039.718462
AAAAAGG588550.039.66966670
AAAAGGG205800.038.24679670
GTCGGAA44850.038.175262
CGTGGAA116550.037.7762961
GGTGGAA149200.037.6962461
CCGTCGT163150.037.21888751
TATGCCG494450.036.86375447
ATGCCGT378850.036.86010448
GTGGGCG91300.036.8386456
GTATGCC518500.036.4228746
GCGTGAA176050.036.1814860
TGGGGGT149750.036.13213757
CGTATGC507050.036.01672745
TCGGCGT87300.035.96078557
ATGCCGG145750.035.7790448
Produced by FastQC (version 0.11.5)