FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8330960
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGGTG478860.5747957018158772TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGATG371990.4465151675197096TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGTTG338710.4065677905067363TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATG311260.3736184065221775TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGTTG254400.3053669685126324TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGATG228920.27478225798707473TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGGTG151520.18187579822733516TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG141080.16934422923648654TruSeq Adapter, Index 5 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTTTG129890.15591240385261723TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGTATG100910.12112649682629612TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGGTT92130.11058749531866675TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGTTTG91020.10925511585699607TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGTTT89800.10779069879101569TruSeq Adapter, Index 5 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA40000.050.172511
AAAAAGG619300.049.4599970
ATGCCGG113250.042.9251548
GGGTGGA237850.042.77522760
GCGTGGA72350.042.03699560
GGTGGAA251400.041.6948161
GTGGAAA326650.041.59329262
AAAAAAG780750.041.43771469
TATGCCG97700.039.9776747
GTCGGAA48250.039.4071042
GTATGCC104000.039.137646
GGATGCC81600.038.81559446
CGGATGC62850.038.03323445
GATGCCG73250.037.93680247
GCCGGCG70600.037.72509850
GCGGATG90450.037.3780344
CCGGCGT70300.037.0397551
CGTATGC93600.036.8305545
GATCGCG60650.036.5852740
TGCCGGC78050.036.4108649
Produced by FastQC (version 0.11.5)