FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9082613
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2406402.6494578157188906TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTG187390.20631727895925986TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC152520.16792524353949684TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATG97650.10751311324175103TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATG92950.10233839094542507TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTGTG90980.10016941159994375TruSeq Adapter, Index 4 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA32600.042.5382461
GGGTGGA87650.041.5278560
GCGTGGA78200.040.14613760
GCCGTCG151800.038.94178850
AAAAAGG488450.038.1987470
GTGGAAA187850.036.98344462
CCGTCGT160600.036.9171651
TGGGGGT95900.036.3495357
AAAAGGG174850.035.970270
TATGCCG495050.035.80161747
ATGCCGT413200.035.43964848
GTGGGGG111000.035.40909256
GCGTGAA163550.035.35228760
GTATGCC515800.035.32489446
CTCGTAT517700.035.2626643
TCTCGTA520800.035.1535742
TCGGCGT77500.035.13467457
TGCGTGA84600.034.99919559
CGTATGC516700.034.972145
GGGGGTG99000.034.89313558
Produced by FastQC (version 0.11.5)