FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6414113
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTG187280.2919811359731268TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTG170380.26563298775684185TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTG128150.19979379845662215TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTG127780.1992169455075082TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTT81450.12698560190629632TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATG78140.12182510660476359TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTT73570.11470019315219424TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTT71870.11204978771031941TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTG64500.10055950058877978TruSeq Adapter, Index 3 (97% over 45bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA97250.044.15770360
AAAAAGG317300.043.63527370
AGTCGGA23350.041.9929731
TCGGGGA22000.040.24846642
GGTGGAA111600.039.48326561
CGGGGTG134850.038.46349752
CCGGGGT42000.037.581951
GTGGAAA141100.037.4790862
TCGCGGA20200.037.42431642
CATCGGG141050.037.36830540
CATCGCG71050.037.2399640
ATCGGGG113650.037.07733541
TGGCGGG114400.037.04841649
ATGCCGG29300.037.02930548
CGGGGAT22250.036.9648843
CGCGGAT18150.036.83055543
GCGGGGT107550.036.77219451
ATCGCGG45600.036.76395441
AGGCATC273650.036.32238837
GGCATCG227600.036.25960538
Produced by FastQC (version 0.11.5)