FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998633
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTG444290.49373054773986225TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTG401110.44574548156369975TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATG213400.23714713112536093TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTTTG121900.13546502007582706TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTATG99830.11093907263469906TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATG99760.11086128304154642TruSeq Adapter, Index 2 (97% over 45bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA136100.043.09905660
AAAAAGG390000.042.21381870
ATGCCGG44550.041.3228248
AGTCGGA30650.039.533741
CGGGGAT50450.039.05702643
TCGGGGA53700.038.58329442
TGCCGGG74100.038.5410849
GGTGGAA155350.038.5019561
GTGGAAA200150.037.0708262
GCCGGGG76850.035.97784450
TCGCGGA29200.035.7178542
CGGGGTT103600.035.30274243
GCGTGGA39750.035.1307760
CCGGGGT69300.035.04920251
TGGCGGG165250.034.64924249
AAAAAAG504550.034.57903769
TGGGGGT143400.034.55938757
GCCGGCG32650.034.19474850
CGCGGAT27450.034.04245443
CCGGCGT32900.034.04128651
Produced by FastQC (version 0.11.5)