FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8885968
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTG336780.3790020400703671TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGTTG253900.2857313913351927TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGTTT95810.10782168020411506TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTT93720.10546965732939845TruSeq Adapter, Index 20 (97% over 41bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA27250.044.0789831
GGGTGGA90050.041.31465560
AAAAAGG350700.039.24030370
CGGGGTG122450.035.24186752
GCGGGGT102050.034.1588651
GTCGGAA34600.033.593362
GGTTGGC71300.033.2319846
TGGCGGG133900.033.1955749
AGATCGG529150.033.116351
GTTGGCG61800.033.07359347
GGTGGAA115750.032.95794361
TTGGCGG72400.032.05029348
GATCGGA549600.031.93322
AAAAAAG464150.031.51900769
GGCCATC98850.031.1219537
CATGGGG268700.030.55729940
ATGGGGG245700.030.46914341
ATCGGGG32300.030.44797341
CATCGCG36600.030.21764640
CATCGGG40600.030.08527640
Produced by FastQC (version 0.11.5)