FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7178658
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1294971.8039165537625554TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA216450.30151875183356TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC99320.13835455039089478TruSeq Adapter, Index 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA20450.040.928461
GCCGTCG86500.035.2009750
GCGTGAA89550.034.08045660
CGGCGTG47350.033.7053958
TCGGCGT45600.033.69412657
GGCGTGA44350.033.3022359
GATCTCG312450.032.7415940
CTCGTAT309650.032.55164343
CCGTCGT94550.032.5003151
CGATCTC314550.032.47849739
ATGCCGT299600.032.39362348
GTATGCC325500.032.3858646
TATGCCG321650.032.3165147
GCGTGGA31700.032.23874360
TGCCGTC266650.032.13078349
CGTATGC327750.032.07810645
TCTCGTA310950.032.0103642
ATCTCGT314100.031.94561641
CACGATC333450.031.81315437
TCGTATG326650.031.6075544
Produced by FastQC (version 0.11.5)