FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9175574
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGGTG956351.0422781179684235TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGTTG921441.0042314518960884TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGATG377180.4110696507924191TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGTTT274130.29876060069920424TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGTTTG268950.29311517731751713TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGGATG254980.27788997178814095TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGGTTG240840.26247949174623847TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGGTT232210.2530740856103389TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGTATG213170.23232334020738105TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGTATG183650.2001509660322068TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATG173070.18862035225262203TruSeq Adapter, Index 19 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGTTTG149570.16300887552103008TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGGGG111820.12186703523943025TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGTTG110250.12015597062374518TruSeq Adapter, Index 19 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGATT104070.11342069716837333TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGTATG91780.1000264397627876TruSeq Adapter, Index 19 (97% over 43bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA58100.057.024061
AAAAAGG903950.051.4013870
GTCGGAA63150.050.5104872
GGGTGGA332050.048.32677560
ATGCCGG90950.048.2170148
GGTGGAA348100.047.17434761
GTGGAAA427100.046.29891262
CGGGGAT79350.045.60637343
TCGGGGA87700.044.25719542
GCGTGGA75350.043.75418560
AAAAAAG1136250.043.56317569
CCGGGGT142700.042.9451551
GGATGCC68800.042.37487846
CGCGGAT55400.042.20059643
TCGCGGA61200.042.0899442
GTTGGCG146700.041.75032847
GATGCCG57750.041.57421547
TTGGCGG189650.041.39313548
TATGCCG73950.041.22226747
TGGAAAA510350.041.18099263
Produced by FastQC (version 0.11.5)