FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7104782
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGGTG89940.12659079476330168TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGTTG78830.11095343952847532TruSeq Adapter, Index 18 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA45900.036.2954560
TGGCGGG46250.034.35595349
GCGGGGT41050.032.9955851
CGGGGTG58950.032.8321452
TGGGGGT47300.032.26140657
CGTCCGC187700.031.88524234
ACGTCCG189250.031.79053933
GTCCGCA186300.031.7303435
GTGGGGG71150.031.72800456
ATCGGGG66700.031.43105141
CATCGGG88550.031.14551740
CGCATCG137100.031.01678338
AGTCACG197900.030.84314229
GCATCGG91550.030.77481539
AGATCGG214700.030.5961361
GTCACGT200100.030.381630
TCACGTC199250.030.37068631
CACGTCC198850.030.36137432
GATCGGA218550.030.1462782
CCGCATC182400.030.10620537
Produced by FastQC (version 0.11.5)