FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8964776
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG1633551.8221871912917846TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATGTCGTATG152880.1705340992346044TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATG135970.15167138587734932TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC127010.14167671339473512TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA28250.041.030821
GCCGTCG124550.040.3800250
GCGTGGA64750.040.16081660
GGGTGGA70250.039.65703660
CCGTCGT133550.038.31395351
TCGGCGT67750.037.9175557
GTGGGCG51550.037.81638756
GCGTGAA131900.037.78493560
GATCTCG367350.037.18536440
TATGCCG386300.037.11887447
CTCGTAT336750.037.0202943
ATGCCGT350550.036.950748
TGCCGTC288250.036.340549
GTATGCC400450.036.340446
GTCGGCG48600.036.2230756
TGCGTGA65050.036.1017959
GCCGGGG44750.036.05465750
CGTATGC392900.035.99650645
GGGCGTG58200.035.7805958
CTGCGTG61650.035.76522458
Produced by FastQC (version 0.11.5)