FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8275585
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG48850.032.9567952
GCGGGGT39600.031.90536151
TGGCGGG48200.031.07766349
GGGTGGA43900.030.69360460
ACCCGTC168100.029.85610433
TCACCCG171100.029.65989931
CACCCGT171900.029.39970632
CCGTCCA166350.029.20238735
CCCGTCC169600.029.17932534
CGTCCAT155950.029.15247536
GTCACCC181300.028.37730630
GTCCATC74650.027.98956737
GGCGGGG56250.027.75006550
ATCGGGG29650.027.73925641
GTGGGGG69450.027.61594856
CAGTCAC193100.027.47695428
CATCGGG37900.027.3340640
AGTCACC191150.027.26290129
GAACTCC202700.027.15980522
GCACACG205800.027.15883812
Produced by FastQC (version 0.11.5)