FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8204561
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG1202321.4654288023454272TruSeq Adapter, Index 15 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA299240.3647239626836829TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCGCGTATG165130.2012661006481639TruSeq Adapter, Index 15 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTTTG89020.10850062544479833TruSeq Adapter, Index 15 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC85080.1036984184772348TruSeq Adapter, Index 15 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA68200.039.5662360
GCCGTCG87250.038.2681650
AGTCGGA23400.037.864251
GCGTGGA56400.037.2949560
CCGTCGT92550.036.87082351
TATGCCG274400.036.72080647
ATGCCGT222550.036.29634548
GGGCGTG54100.035.51646858
GTATGCC293000.035.36921746
GTCGGCG37800.034.9063356
CGTATGC288150.034.88353345
CTCGTAT273100.034.7936643
GTGGGCG50500.034.5830956
TCGTATG275100.034.4898244
GCGTGAA96900.034.4571460
TGCCGTC182900.033.8890649
GGCGTGA55050.033.88633359
TCTCGTA282250.033.82691642
TGGGCGT59100.033.63686857
ATGCCGG79100.033.5830448
Produced by FastQC (version 0.11.5)