FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8640960
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA244400.2828389438210569TruSeq Adapter, Index 14 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA75200.036.34829760
AGTCGGA21050.030.9462721
AAAAAGG254600.030.83342670
TGCCGGG48150.030.45570249
CGGGGTG103150.030.23147652
CCGGGGT46050.029.94452751
ATGCCGG40350.029.9244748
GCCGGGG48600.029.8856550
GCGGGGT71000.029.32982651
CATCGCG38750.029.2633640
GTGGGGG119300.028.5738956
GGTGGAA100800.028.08933361
TATGCCG55000.027.80799347
GCGTGGA30550.027.3802660
GGTGGGG109400.027.09673555
AGATCGG468300.026.6539171
GTGGAAA138750.026.5864962
GTATGCC61150.026.49936346
GGGTGGG121250.026.49792354
GATCGGA472900.026.4031942
Produced by FastQC (version 0.11.5)