FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8313002
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG746460.897942764839946TruSeq Adapter, Index 13 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTTTG103510.12451578864049354TruSeq Adapter, Index 13 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGATG87920.1057620339800231TruSeq Adapter, Index 13 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGTATG83830.10084203035197153TruSeq Adapter, Index 13 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA71350.038.6530960
GCGTGGA48150.036.56147460
TATGCCG166000.035.35713247
AGTCGGA26050.035.2229081
GCCGTCG58200.035.05884650
GTATGCC179400.034.1208246
ATGCCGT138850.034.07868648
AAAAAGG318350.033.57501670
CGTATGC177600.033.46160545
GGGCGTG49200.033.3625958
ATGCCGG60250.033.3431748
AGATCGG437850.033.297581
GCCGGGG51350.032.91991850
CTCGTAT177150.032.8748843
GTGGGGG103800.032.84079456
CCGTCGT64000.032.75660351
TGGGGGT87000.032.58500357
TGCCGTC109850.032.37022449
TGCCGGG56550.032.3683949
GATCGGA451050.032.323112
Produced by FastQC (version 0.11.5)