FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8942471
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG440040.4920787554133528TruSeq Adapter, Index 12 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG273650.3060116158050722TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGGTG216110.24166698443864118TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGTTG162620.18185130262094223TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTATG162450.18166119856581028TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATG161530.180632400149802TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGTTG143850.16086157841607762TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTTTG123780.13841811731902737TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTTTG123300.13788135292806653TruSeq Adapter, Index 12 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGATG119550.13368788112368496TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTG100360.11222848807672958TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGGTG99430.11118850706924295TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGGATG91580.10241017275873748TruSeq Adapter, Index 12 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA37800.047.7111241
AAAAAGG609200.045.3169970
GGGTGGA203650.045.11271360
GCGTGGA76300.042.98026760
ATGCCGG106800.042.24114648
GGTGGAA227200.041.9156961
GTGGAAA309250.041.8291262
GTATGCC164700.039.16388346
TATGCCG154750.039.10374547
GCCGGCG58400.038.83436250
AATCGCG108750.038.74817340
ATCGCGG50550.038.6337741
GTCGGAA44800.038.449592
AAAAAAG764000.038.223969
CGTATGC158150.038.01963845
CCGGGGT124350.037.2082951
TGTAATC365550.037.12915437
TCGCGGA26850.037.0192942
CGCGGAT24350.036.9392643
TGCCGGC69400.036.91523449
Produced by FastQC (version 0.11.5)