FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6916903
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1316711.9036120645323493TruSeq Adapter, Index 11 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATG120420.17409525621510089TruSeq Adapter, Index 11 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC110120.15920419875773884TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTTTG98740.14275174886795433TruSeq Adapter, Index 11 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA23200.039.847021
GGGTGGA55900.039.56936660
GCCGTCG84200.039.0307950
GCGTGGA49650.037.78325360
GGCGTGA49350.036.45270559
GTGGGCG39800.036.3179156
TATGCCG275900.036.2673947
GCGTGAA95700.036.02285460
AGATCGG453400.035.8975261
GTATGCC286900.035.70639446
GCCGGGG36750.035.4274150
TGCCGGG42000.035.33217649
CCGTCGT94750.035.20201551
ATGCCGT232400.035.14945248
CATCTCG321750.035.04780640
CGTATGC288800.035.0109745
AAAAAGG307300.034.96468770
CTCGTAT278600.034.89835743
TGCCGTC192900.034.6541349
TCTCGTA282200.034.58958442
Produced by FastQC (version 0.11.5)