FastQCFastQC Report
Fri 20 Nov 2020
HHGW2AFX2_n01_ncm11-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGW2AFX2_n01_ncm11-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8105152
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTG498420.6149421997267911TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTG388160.47890526914239234TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATG134210.16558603712798972TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGG99500.12276142384498157TruSeq Adapter, Index 10 (97% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA86150.044.6881160
AGTCGGA25850.042.1319661
AAAAAGG311900.041.31619670
TGGCGGG117250.039.19257449
CGGGGAT33600.039.0610543
TCGGGGA37000.038.9715342
TGCCGGG37000.037.5526649
CGGGGTT89000.036.37505743
GGTGGAA107100.036.3389761
GCGGGGT93650.035.9143151
CGGGGTG117400.035.5651252
TCGGGGT96950.035.5221542
GCCGGGG42800.035.48933850
TGGGGGT86850.035.42204757
GTTGGCG57950.034.4249547
TTGGCGG65300.033.98036248
GGTTGGC64000.033.7956246
GTGGAAA132850.033.27354462
AGATCGG476350.033.207471
ATCGGGG301850.033.05658341
Produced by FastQC (version 0.11.5)