FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18951110
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233707712.332137800899261No Hit
GGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192930.10180406319207688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAT19050.010.4106471
CCGGGAT34500.010.2035661
CGGGAAT29600.09.8826421
CCGGTTT28600.09.5347521
CCGGTAT12459.094947E-129.1594891
GCGGAAT20100.08.8801271
CTCGGAT39500.08.6609341
GGGAAAT89050.08.6299941
CGGGGAT31800.08.4193661
CGGGCAT32950.08.1255191
CGGAAAT32450.08.0979281
GGGGAAT58750.08.0173061
CGGGATT21650.07.99836832
CGCCGAT18900.07.8699541
CCGGAAT29550.07.71814731
AGATCGG186400.07.62800994-95
CGCAAAT22200.07.59344241
CGGAATT26550.07.46978661
CCGGCAT30250.07.37564231
AATCGAC18303.6379788E-127.3027995
Produced by FastQC (version 0.11.5)