FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_FDK263_LDI4_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_FDK263_LDI4_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38458555
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT97750.010.7027091
CGGGGAT103250.010.3628751
CGGCAAT56150.09.4007181
GCGGAAT56600.09.1579421
CCGGGAT98600.09.0671071
CGCGGAT30900.08.9260091
CGGGCAT108450.08.7697711
CGACAAT50650.08.6376611
CTCGAAT93850.08.6139531
CGAATGT82300.08.5421723
CGGAATT76300.08.5320812
CGCAAAT46500.08.3858811
CGGGAAT91650.08.3537451
GGCGTAT52750.08.2937931
GGGAAAT264700.08.2460611
GTCGGTT73200.08.055611
GGCGAAT49600.08.05351451
CGGAAAT96350.08.0449561
GCCGGAT67850.07.9899271
AATCGAG47650.07.97453365
Produced by FastQC (version 0.11.5)