FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_FDK261_LP6_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_FDK261_LP6_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22058911
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT57500.010.5044561
CCGGAAT58050.09.831431
CGAATGT47500.09.7001823
CGGCAAT33500.09.6538771
CGGGAAT55350.09.1939851
CTCGAAT55050.09.0713021
CCGGGTT43700.09.0330491
CGGGCAT59900.08.8132041
GTTCGAT34000.08.8125031
CCGGGAT58950.08.7938771
CGGCGAT25500.08.7658771
CGGAAAT53100.08.68790151
AATCGTA24900.08.5839745
GTCGGTT41600.08.5744371
CGACAAT26950.08.4707161
CGCGGAT18050.08.4316091
CGACGAT21500.08.4058721
GCGGGTT34550.08.3969091
CCGGTTT46500.08.3868441
GGGAAAT155650.08.2194141
Produced by FastQC (version 0.11.5)