FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_FDK256_LDI2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_FDK256_LDI2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29698824
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT41350.010.1215641
CCGGAAT73050.09.7007781
CGGGGAT76650.09.5554041
GCGGAAT41650.09.4777141
AATCGAG38500.09.376225
CGCGGAT25400.09.3621751
CCGGGAT79700.09.309081
CGGGAAT66100.08.7780671
GCCGGTT56850.08.7004821
AAATCGA53850.08.6440394
CGGAAAT70050.08.6225571
CTCGAAT71300.08.5380941
CGGAATT56300.08.5250532
GCGGATT56150.08.4701421
GGGGAAT143850.08.430841
GGAAAAT291450.08.4202721
GTTCGAT40400.08.3582921
GCGGGAT74150.08.3382071
GGCGAAT37450.08.2547141
GGGAAAT215350.08.2376711
Produced by FastQC (version 0.11.5)