FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_FDK255_LP5_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_FDK255_LP5_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18681155
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA119700.010.4361384
CGGGAAT39750.010.288731
GCCGAAT27850.09.7330761
CGGCGAT21300.09.1538691
CGACAAT25750.09.049391
ATCTACA131550.08.8462746
CATCTAC135000.08.8312165
CCGGGAT50700.08.816991
CGGCAAT28200.08.7690931
GGGAAAT139400.08.76741
CGGGGAT48700.08.5931851
CCCACAT156050.08.4719131
GGCGAAT23650.08.4453691
CGACGAT17650.08.3525191
CGCCGAT33800.08.3011031
GTCGGTT30650.08.2232941
GATCGGA248950.08.21414894-95
GCGAAAT17450.08.1757251
ATCGGAA237650.08.17498594-95
GCGTCGT96800.08.14580994-95
Produced by FastQC (version 0.11.5)