FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_FDK254_LP3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_FDK254_LP3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19716794
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT21850.09.7948781
GTCGGAT27050.09.6701541
CGGGGAT52000.09.5119151
CGGGAAT42650.09.366951
GGCGAAT23150.09.2448421
CCGGGAT54950.08.9147161
GCCGAAT30600.08.7037121
CGGGTAT22050.08.6275871
CGGCAAT30000.08.5607231
CGAATGT41100.08.4367813
CTCGAAT49050.08.4356431
AATCGTG27650.08.4174215
CGCAATT21500.08.4058781
CGGGATT33500.08.3761641
CGCGGAT17100.08.3437851
CCGGAAT52450.08.2515181
GGCGTAT25650.08.1583681
CGCCGAT22800.08.1351911
GGGGAAT96350.08.0952471
CTCGGAT64650.08.0921161
Produced by FastQC (version 0.11.5)