FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_FDK239_LP4_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_FDK239_LP4_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18426524
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAT23650.010.2546431
ACATCTA116400.09.793414
GGCGAAT24400.09.7445481
CGGGGAT50200.09.6622441
CGGGAAT42600.09.3767251
CGAATGT33950.09.0947443
GCGGAAT23550.09.0866371
GGGAAAT138900.08.6273981
CGGCAAT26350.08.482011
GCGGGTT28150.08.4464291
GGGGAAT86550.08.4063191
ATCTACA129750.08.2733066
GTCGGTT31650.08.2636231
CGAAAAT37500.08.2425891
GGAAAAT175050.08.2311781
CGGGCAT52750.08.2035251
CGCGGAT18050.08.1670651
CTCGGAT58250.08.00041
GATCGGA245250.07.9605194-95
CATCTAC132800.07.86865045
Produced by FastQC (version 0.11.5)