FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_FDK237_LDI3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_FDK237_LDI3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31850461
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT83700.011.2500611
CGGGGAT80500.010.9292651
GGCGTAT43950.010.49611
CGCCGAT65350.010.4065211
CGGCAAT46750.010.1726361
CGCGGAT27950.010.0388811
CGACGAT30800.09.7275831
GTTCGAT46300.09.6552161
CGGGAAT72300.09.6035021
CGGCGAT36350.09.4198331
CGCAAAT41950.09.296021
ACATCTA218700.09.2085784
GCGGAAT45700.08.9494711
GTCGGAT38350.08.5565531
CGGGCAT82450.08.5366251
CCGGGAT86050.08.5110861
CGGAAAT80000.08.4413811
CGGAATT67150.08.4211512
ATCTACA214900.08.3989856
GAATCGA42550.08.3721544
Produced by FastQC (version 0.11.5)