FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_FDK218_LDI1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_FDK218_LDI1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22955455
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA148100.09.8139074
CGGGGAT60850.09.5344451
CGCCGAT45150.09.3740751
GCCGGAT43700.09.14100551
CGGCGAT24050.08.8980251
CGGAAAT53800.08.8392191
CCGGGAT65450.08.791681
ATCTACA163650.08.7073036
CGGGCAT64900.08.5730891
CGGCAAT33900.08.5570941
CTCGAAT54700.08.5199081
CATCTAC170100.08.4329425
CGCGAAC13005.820766E-118.4135761
CCGGTTT58900.08.3968081
CGGGAAT50650.08.3561591
CCCACAT190850.08.3224361
GGCGAAT28300.08.2339041
CGCAAAT26900.08.1320811
GCGAAAT20500.08.1191461
TCGAATG46950.07.99560932
Produced by FastQC (version 0.11.5)