FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_FDK216_LP2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_FDK216_LP2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14851455
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT13450.011.313931
CGGCAAT24450.011.2806711
CGGGGAT40400.011.0645131
CCGGGAT43200.010.3473681
ACATCTA92800.010.2878884
GCCGAAT24700.010.2038661
CGGGAAT34700.010.141171
AATCGAG20350.010.0363625
CGCGTTT9459.404175E-109.5610941
CTCGAAT32250.09.4370451
GGGAAAT114650.09.0006011
GTTCGAT20700.08.9594231
AAATCGA29500.08.8556174
CGGAAAT36150.08.8135791
CGACGAT15700.08.7838341
CGGCGAT18050.08.4306011
CGGGCAT39050.08.4026021
CGAGAAT27700.08.240381
CGTCGTG73550.08.20210894-95
CCGGAAT35450.08.1827521
Produced by FastQC (version 0.11.5)