FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_DJA270_DOM2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_DJA270_DOM2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33617462
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT31900.010.8829471
ACATCTA220800.010.4976834
CGGGGAT91450.09.62060451
CGGGAAT72800.09.6028541
CGGAAAT72900.09.1982661
ATCTACA240250.08.8175926
CGCAAAT38900.08.8023221
GTCGGTT51650.08.7471711
CGGCGAT38000.08.6353481
CATCTAC245700.08.5832645
AATCGAA49100.08.5128665
GATCGGA461750.08.4457494-95
CGGCAAT53200.08.4029261
GTTCGAT42050.08.3691251
ACCTTAC104700.08.347376
CCGGAAT77950.08.2973061
GGGAAAT238550.08.1737021
CGCGAAT20400.08.1592871
ATCGGAA439700.08.1346794-95
GGCGAAT42750.08.1208431
Produced by FastQC (version 0.11.5)