FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_DJA269_DOM3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_DJA269_DOM3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31282667
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT80300.010.4818851
GCGGAAT42150.010.0409331
ACATCTA198950.010.003384
GTTCGAT42750.09.9000071
CCGGAAT71800.09.6034041
CGGGAAT69350.09.3941121
CGGCAAT48250.09.2642911
CGCCGAT43650.08.9332871
CCGGGAT81150.08.731311
GGGAAAT218300.08.6044871
CGAATGT58550.08.5997443
GGCGAAT41250.08.5307941
CGGCGAT31900.08.3479341
CGCGAAT19400.08.3341031
GTCGGTT51500.08.3103051
CGACGAT27600.08.2701581
CGGAAAT68000.08.2519141
GGAAAAT307300.08.15510751
GATCGGA458500.08.15347294-95
AGATCGG519100.08.0938394-95
Produced by FastQC (version 0.11.5)