FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_DJA268_DODI2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_DJA268_DODI2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24269752
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT57100.09.9108521
CGGCAAT36600.09.8748991
CCGGAAT61900.09.7569311
CGAATGT53450.09.331323
CGGGCAT67300.09.2567041
CGACGAT25500.09.13810351
CGATATA24500.09.1158092
CGGGGAT66400.09.0956941
GCGGAAT34300.09.0119631
GGCGTAT35550.08.9626271
TCGGATC55550.08.8963672
GTCGGTT47150.08.875671
GTCGGAT33550.08.7881881
CTCGAAT63900.08.78175451
CCGGGAT67800.08.6974541
GGGGAAT117000.08.6575281
GCCGAAT33000.08.6464421
CCGATAT34700.08.6339841
AATCGAG32150.08.5688865
AATCGTA26250.08.5044435
Produced by FastQC (version 0.11.5)