FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_DJA267_DODI3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_DJA267_DODI3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26551903
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT57800.010.1191261
CGGGTAT28700.09.9411151
GCGGAAT34300.09.7044221
CGGGGAT71800.09.1394571
GGGAAAT189600.09.1291171
CCGGAAT59050.09.01911451
CCGGGAT75150.08.8585941
CGACAAT33300.08.7106661
CGGCAAT40500.08.5710411
ACATCTA167000.08.5325964
AAATCGA50750.08.5169054
AATCGAG40250.08.4965415
CGCGTAA5800.00119820488.1985631
GTCGGTT41300.08.1747421
GCGTCGT136750.08.1457694-95
CTCGAAT57650.08.0833711
GATCGGA344400.07.986040694-95
GGAAAAT253600.07.95027881
CGTCGTG129200.07.886437494-95
CGCGGAT26700.07.83622931
Produced by FastQC (version 0.11.5)