FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_DJA263_DOP3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_DJA263_DOP3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24841746
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT71750.010.0748711
CGCCGAT38750.09.81829551
TAGCGAT26150.09.8085049
CGGCAAT39200.09.70558551
ACATCTA160450.09.6209364
CGGGGAT68350.09.5323471
CCGGAAT58500.09.4301791
CGGGAAT59600.08.93695451
CGGAAAT59250.08.8292151
AATCGAG36850.08.7648635
GTTCGAT34000.08.6722261
GGGAAAT177600.08.6224511
CCGGGAT71450.08.5862851
GCGGAAT34900.08.5848541
CGGCGAT30450.08.4338181
CGAAAAT53200.08.31361
GTCGGAT35550.08.2941121
CGCAAAT31250.08.2179131
CGCGGAT23750.08.2099041
ATCTACA177150.08.2045856
Produced by FastQC (version 0.11.5)