FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_DJA260_DODI1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_DJA260_DODI1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23489810
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA159600.011.8744914
ATCTACA172000.010.5766266
CATCTAC178400.09.7977865
CCCACAT206400.09.4924571
TAGCGAT28550.09.4831579
TACAGAC166900.09.4485689
CGCAAAT28400.09.3769331
CGGGAAT51750.09.3730511
CGCCGAT36000.09.2466971
CGGGTAT25450.08.9690111
CCGGAAT52300.08.819851
GGGAAAT166100.08.4458531
CGGGGAT63850.08.4160581
GTAGCGA33050.08.3355878
CGGGCAT66550.08.288981
GGGGAAT110450.08.2235391
AGATCGG390200.08.21118494-95
GCGGTTT53450.08.1852381
TCTACAG216000.08.1582677
GTCGGTT36800.08.1411151
Produced by FastQC (version 0.11.5)