FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_DJA258_DOP1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_DJA258_DOP1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27669589
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT70750.09.7463971
CGGGGAT73300.09.6668471
GCGGAAT38850.09.4254541
GTTCGAT36900.09.27916051
GCCGAAT38450.09.2761431
CGACGAT29550.09.1731811
CGAAAAT68000.08.8817261
CGGAATT54300.08.7512422
CGGCGAT29950.08.7330991
CGCGGTT26900.08.6625621
GTCGGTT50400.08.4920881
GCGGGAT69750.08.4543461
CGCGATT10708.782081E-98.4444691
CGATATA25750.08.3043342
TTCGATT45000.08.2366692
AATCGAG36450.08.2095375
CGCAAAT34950.08.1640681
GGGGAAT128950.08.1502981
GATCGGA366800.08.13916494-95
GTCGGAT37400.08.1378731
Produced by FastQC (version 0.11.5)