FastQCFastQC Report
Fri 12 Jun 2020
HHGL7BGXF_n02_DJA248_DOM1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGL7BGXF_n02_DJA248_DOM1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33146360
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA232350.012.531284
ATCTACA252000.010.8190786
CATCTAC257950.010.643165
CCGGAAT76000.010.3867451
CGGCGAT39900.010.1305041
CCCACAT288800.09.8301951
TACAGAC246700.09.8001529
CGGCAAT48950.09.7147661
AAATCGA63450.09.6568554
CGGGGAT88100.09.4459831
CGGGAAT71700.09.4179031
CGCGTAT10851.2551027E-109.2039571
TAGCGAT41900.08.842299
AATCGAG45800.08.8151915
ACCTTAC103200.08.422686
CGGAATT57100.08.4055232
CGCCGAT45850.08.4009941
GCGGAAT42550.08.3819821
CACATCT366550.08.3326833
CGGGCAT91950.08.3264361
Produced by FastQC (version 0.11.5)